Crispr/Cas9 腺病毒


Crispr/Cas9 简介

CRISPR (clustered, regularly interspaced, short palindromic repeats)是一种来自细菌降解入侵的病毒 DNA 或其他外源 DNA 的免疫机制。目前,来自Streptococcus pyogenes 的CRISPR-Cas9系统应用最为广泛。Cas9 蛋白(含有两个核酸酶结构域,可以分别切割DNA 两条单链。)   Cas9首先与crRNA及tracrRNA结合成复合物,然后通过PAM序列结合并侵入DNA,形成RNA-DNA复合结构,进而对目的DNA双链进行切割,使DNA双链断裂。


通过基因工程手段对crRNA和tracrRNA进行改造,将其连接在一起得到sgRNA(single guide RNA)。融合的RNA具有与野生型RNA类似的活力,但因为结构得到了简化更方便研究者使用。通过将表达sgRNA的原件与表达Cas9的原件相连接,得到可以同时表达两者的质粒,将其转染细胞,便能够对目的基因进行操作。











   1、Laterodorsal tegmentum interneuron subtypes oppositely regulate olfactory cue-induced innate fear;     (2016年发表于Nature Neuroscience,作者:段树民)   2、Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury Involvement of PARK2-dependent mitophagy;     (2016年发表于Autophagy作者:Xiangnan Zhang)   3、SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain;     (2016年发表于Nature Communications,作者:陈义汉)   4、TRB3 links insulin IGF to tumour promotion by interacting with p62 and impeding autophagic proteasomal degradations;     (2016年发表于Nature Communications,作者:胡卓伟)   5、Suppression of KRas-mutant cancer through the combined inhibition of KRAS with PLK1 and ROCK;     (2016年发表于Nature Communications,作者:刘明耀)   6、Synergized regulation of NK cell education by NKG2A and specific Ly49 family members     (2019年发表于Nature Communications,作者:Zhang X)   7、LRP6 acts as a scaffold protein in cardiac gap junction assembly;     (2016年发表于Nature Communications,作者:陈义汉)   8、Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression;     (2016年发表于plos genetics,作者:Jun Yan )   9、Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy;     (2016年发表于Kidney International,作者:易凡)   10、Roles of miR-1-1 and miR-181c in ventricular septal defects;     (2016年发表于International Journal of Cardiology,作者:Li J)   11、Intronic SMCHD1 variants in FSHD: testing the potential for CRISPR-Cas9 genome editing;     (2019年发表于J Med Genet,作者:Balog J)   12、Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9;     (2019年发表于Stem Cell Research ,作者:Terbto P)   13、Liposomal delivery of CRISPR/Cas9;     (2019年发表于Cancer Gene Therapy,作者:Zhen S)   14、Brain transcriptome profile after CRISPR-induced ghrelin mutations in zebrafish;     (2019年发表于Fish Physiological and Biochemistry,作者: Soletto L)   15、CRISPR/Cas9-mediated gene correction in hemophilia B patient-derived iPSCs;     (2019年发表于Int J Hematol,作者: Seki R)   16、A CRISPR/Cas9-based strategy to simultaneously inactivate the entire gene family in Candida orthopsilosis;     (2019年发表于Future Microbiolgy,作者:Bottai D)   17、Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system;     (2019年发表于Cell Death&Disease,作者:Chen C)   18、A CRISPR-Cas9 system for multiple genome editing and pathway assembly in Candida tropicalis;     (2019年发表于Biotechnology and Bioengineering,作者:Shen W)   19、MicroRNAs as Diagnostic, Prognostic, and Therapeutic Biomarkers in Prostate Cancer;     (2019年发表于Crit Rev Eukaryot Gene Expr,作者:Amiri A)   20、MicroRNA regulation of CTP synthase and cytoophidium in Drosophila melanogaster;     (2019年发表于Experimental Cell Research,作者:Dzaki N)   21、External validation of a panel of plasma microRNA biomarkers for lung cancer;     (2019年发表于Biomakers in Medcine,作者:Li J)   22、Kinetics Analysis of Circulating MicroRNAs Unveils Markers of Failed Myocardial Reperfusion ;     (2019年发表于Clinical Chemistry,作者:Ali S)   23、Cumulus cell-derived and maternal SIRT6 differentially regulates porcine oocyte meiotic maturation;     (2019年发表于Theriogenology,作者:Yu T)   24、Sirtuins and SIRT6 in Carcinogenesis and in Diet;     (2019年发表于Internationl Journal of Molecular Science,作者:Souto EB)   25、Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy;     (2016年发表于International Journal of Molecular Medicine,作者:Hong Wang)   26、Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats;     (2016年发表于Spinal Cord)   27、miR-23b-3p regulates the chemoresistance of gastric cancer cells by targeting ATG12 and HMGB2;     (2016年发表于Cell Death and Disease,作者: Q Zhao)   28、Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes;     (2016年发表于Osteoarthritis and Cartilage,作者: Jian Zhang)   29、β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy;     (2016年发表于 Cell Death and Disease,作者:F Yi )   30、MicroRNA-30a downregulation contributes to chemoresistance of osteosarcoma cells through activating Beclin-1-mediated autophagy;     (2016年发表于Oncology Reports,作者:Lifeng Lao)   31、Luteolin alleviates post‐infarction cardiac dysfunction by up‐regulating autophagy through Mst1 inhibition;     (2016年发表于J. 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Med.,作者:Dongdong Sun )   32、Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p;     (2016年发表于Biochemical and Biophysical Research Communications,作者: Jiahong Dong)   33、MST1 suppresses viability and promotes apoptosis of glioma cells via upregulating SIRT6 expression;     (2019年发表于Journal of Integrative Neuroscience,作者:Zhu D)   34、Long non-coding RNA HNF1A-AS1 functioned as an oncogene and autophagy promoter in hepatocellular carcinoma through sponging hsa-miR-30b-5p;     (发表于Biochemical and Biophysical Research Communications,作者:Jiahong Dong)   35、miR-186 inhibits cell proliferation in multiple myeloma by repressing Jagged1;     (2016年发表于Biochemical and Biophysical Research Communications,作者:Jun Peng)   36、House dust mite extract induces growth factor expression in nasal mucosa by activating the PI3K/Akt/HIF-1α pathway;     (2016年发表于Biochem Biophys Res Commun,作者:Zhou H)   37、Sitagliptin inhibits vascular inflammation via the SIRT6-dependent signaling pathway;     (2019年发表于International Immunopharmacol,作者   Zhao F)   38、Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1;     (2016年发表于Oncotarget,作者:Heng Ma)   39、A novel role of microRNA 17-5p in the modulation of circadian rhythm;     (2016年发表于Sci Rep,作者:Ji-Min Cao )   40、Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease;     (2016年发表于J Autoimmun,作者:Xiao Y)